Letter to the Editor
Real-time PCR for Quantification of Helicobacter felis in Mouse Stomach
Abstract
Helicobacter pylori is the most common cause of chronic bacterial infections, affecting at least 50% of the world's population and leading to gastritis, peptic ulcer disease and gastric adenocarcinoma. Development of a vaccine to control this infection has become a priority, and preclinical testing of potential products includes inoculation of mice with H felis and determination of the number of bacteria in the stomach.1 Since the traditional quantitative culture technique is laborious,2 we—as well as others3,4—have developed a real-time polymerase chain reaction (Q-PCR) for this task. Here, we describe our assay.This content is limited to qualifying members.
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