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SMJ // Article

Original Article

Comparison of Superoxide Dismutase Activity in Saliva of Smokers and Nonsmokers

Authors: Maryam Baharvand, MD, Ahmad Ghaem Maghami, MD, Somayyeh Azimi, MD, Hosein Bastani, PhD, Azadeh Ahmadieh, MD, Mehrdad Taghibakhsh, MD

Abstract


Background: Considering the role of superoxide dismutase (SOD) as one of the most important salivary antioxidant enzymes, this study was performed to compare the level of this enzyme in smokers and nonsmokers.


Methods: Unstimulated saliva of 60 volunteers (30 smokers and 30 nonsmokers) was collected. The activity of salivary SOD was measured in each group and compared.


Results: The mean value of superoxide dismutase activity was significantly higher in the smoking group (P < 0.001), while no detectable activity level was found in nonsmokers.


Conclusion: Cigarette smoke leads to an elevation in salivary superoxide dismutase activity.



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References

1.Kanehira T, Shibata K, Kashiwazaki H, et al. Comparison of antioxidant enzymes in saliva of elderly smokers and nonsmokers. Gerodontology 2006;23:38–42.
 
2.Franco EL, Kowalski LP, Oliveria BV. Risk factors for oral cancer in Brazil: a case-control study. Int J Cancer 1989;43:992–1000.
 
3.Llewellyn CD, Johnson NW, Warnakulasuriya KA. Risk factors for oral cancer in newly diagnosed patients aged 45 years and younger: a case-control study in southern England. J Oral Pathol Med 2004;33:535–532.
 
4.Jahanbani J. Prevalence of oral leukoplakia and lichen planus in 1167 Iranian textile workers. J Oral Dis 2003;9:302–304.
 
5.Tobacco use and the periodontal patient. J Periodontil 1996;67:51–56.
 
6.Krall EA, Dawson-Hughes B, Garvey AJ. Smoking, smoking cessation and tooth loss. J Dent Res 1997;76:1653–1659.
 
7.Raveendran M, Senthil D, Utama B. Cigarette suppresses the expression of P4H alpha and vascular collagen production. Biochem Biophys Res Commun 2004;323:592–598.
 
8.Church DF, Pryor WA. Free-radical chemistry of cigarette smoke and its toxicological implications. Environ Health Perspect 1985;64:111–126.
9.Michael Pittilo R. Cigarettes smoking, endothelial injury and cardiovascular disease. Int J Exp Pathol 2000;81:219–230.
 
10.Phillips DH. Smoking- related DNA and protein adducts in human tissues. Carcinogenesis 2005;23:1979–2004.
 
11.Dwyer TM. Cigarette smoke-induced airway inflammation as sampled by the expired breath condensate. AM J Med Sci 2003;326:174–178.
 
12.Zappacosta B, Persichillia S, De Solea P, et al. Effect of smoking one cigarette on antioxidant metabolites in the saliva of healthy smokers. Arch Oral Biol 1999;44:485–488.
 
13.Greabu M, Battino M, Totan A, et al. Effect of gas phase and particulate phase of cigarette smoke on salivary antioxidants. What can be the role of vitamin C and pyridoxine? Pharmacol Rep 2007;59:613–618.
 
14.McCord JM, Fridovich I. Superoxide dismutase: An enzymatic function for erythrocuprein (hemocuprein). J Biol Chem 1969;244:6049–6055.
 
15.Reznick AZ, Cross CE, Hu ML, et al. Modification of plasma protein by cigarette smoke as measured by protein carbonyl formation. Biochem J 1992;286:607–611.
 
16.Reznick AZ, Han D, Packer L. Cigarette smoke induced oxidation of human plasma proteins, lipids, and antioxidants. Redox Rep 1997;3:169–174.
 
17.Nagler R, Lischinsky S, Diamond E, et al. Effect of cigarette smoke on salivary proteins and enzyme activities. Arch Biochem Biophys 2000;379:229–236.
 
18.Reznick AZ, Klein I, Eiserich JP, et al. Inhibition of oral peroxidase activity by cigarette smoke invivo and invitro studies. Free Radic Biol Med 2003;34:377–384.
 
19.Stryker WS, Kaplan LA, Stein EA, et al. The relation of diet, cigarette smoking, and alcohol consumption to plasma beta-carotene and alpha-tocopherol levels. Am J Epidemiol 1988;127:283–296.
 
20.Lee BM, Lee SK, Kim HS. Inhibition of oxidative DNA damage, 8-OHdG, and carbonyl contents in smokers treated with antioxidants (vitamin E, vitamin C, beta-carotene and red ginseng). Cancer Lett 1998;23:219–227.
 
21.Nagler RM. Altered salivary profile in heavy smokers and its possible connection to oral cancer. Int J Biol Markers 2007;22:274–280.